BC3-Z300-V2 Basic micro Channel FlowCell

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THIS IS A SINGLE LINE CHANNEL FOR BASIC APPLICATIONS REQUIRING SMALL VOLUME SAMPLE ANALYSIS. COMPATIBLE WITH MULTI-CHANNEL PIPETTES. SINGLE LARGE MICRO RESERVOIR CHANNEL CAN ALSO BE USED AS MICROREACTOR. CHANNEL LENGTH (Y) AND HEIGHT (Z) IS FIXED BUT CHANNEL WIDTH (X) IS VARIABLE. FOR OTHER MICROCHANNEL SIZES, PLEASE ASK FOR CUSTOM FABRICATION.

THE COLUMNS INSIDE FLOW CELL ARE SUPPORTINGS AGAINST VOLUME CHANGE OF FILLED CHANNEL.

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Microfluidics is beneficial for saving reagent consumption. Single-channel devices are suitable for the applications below;

--- Life Sciences: Immunofluorescence assays, Cell-based short time assays
--- Pharmaceutical Sciences: Parallel screening of antibodies or pharmaceuticals,
--- Biotechnology: Live-cell imaging, cell culture under flow,
--- Diagnostic tech: Diagnostic devices for personalized medicine, novel biosensor research
--- Others: Chemical synthesis and screening, cosmetics, food industry, aerospace and more...


DATASHEET

Specification Info
Number of devices per chip 1 device
Material PDMS bonded on microscopy glass. Please also check the "Our Trip to PDMS Chips" blog post.
Bonding technique Oxygen plasma treatment
XY Size of total chip 25x73 mm2 of the chip on 26x76 mm2 of std microscopy glass
Z Height of PDMS chip 3.0 - 4.0 mm
Y Length of microchannels Y length is 52.5 mm.
X Width of microchannels X width is optional and can be 12.0, 11.0, 10.0, 9.0, 8.0, 7.0, 6.0, 5.0,  4.0, 3.0, 2.0, 1.0 mm
Z Height of microchannels 300 micrometers
Microchannel surface area 657,56 mm2 per channel excluding ports for 0.3x52.5 mm2 (XY)
Microchannel volume 197,25 uL per channel excluding ports for 0.3x52.5x0.3 mm3(XY)
Ports on top/edge on Top
Number of ports 2 ports per device
Pitch btw ports and edge-to-port 3 mm and 3.5 mm
Size / Shape of ports 2 mm / Circle
Suitable connector outer diameter 2.0 - 2.5 mm
microChannel geometry Rectangular
The layout of active ports small-layout-bw-bc3
... ...

 


 REQUIREMENTS

Additional Requirements Suggestions
Pumping/Actuation Flow and mixing of the liquids inside microchannels require low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic and piezoelectric pumps may also be suitable but mind the possible relatively high pulsation.
Fittings All fittings in ufluidic.com are suitable for this chip. Any other connectors compatible with pitch and port size is also applicable. Reactive compatibility of the fittings is also important to be considered.
Chemicals It depends on the application of the researcher.
Visualization Epi-Fluorescent Microscopy is widely used together with microfluidic chips.
Other analysis techniques should be determined by user researchers for the experiments where optical changes inside chips do not occur.
... ...

 


LITERATURE 

Application examples from literature

These are some highly sited articles from literature. The chip designs are not identical but working is the same in principle.

Kondrashin et.al. 2013
Measurement of cell respiration and oxygenation in standard multichannel biochips using phosphorescent O2-sensitive probes
DOI: 10.1039/C3AN00658A
Ganguly et.al. 2012
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
DOI: 10.3791/2F4032
 
 


 Kullanım Kılavuzu

 Yakında eklenecektir 
 Nasıl kullanılır - Multimedya
 
 Yakında eklenecektir 
 Akış Testi - Multimedya 


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