BC4-GR1-Z100 Concentration Gradient Formation

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THIS IS A GRADIENT CHANNEL STRUCTURES FOR APPLICATIONS REQUIRING CONCENTRATION GRADIENT ANALYSIS. 

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Microfludics is beneficial for saving reagent consumption. Single channel devices are suitable for the applications below;

--- Life Sciences: Immunofluorescence assays, Cell-based short time assays
--- Parmaceutical Sciences: Parallel screening of antibodies or pharmaceuticals,
--- Biotechnology: Live-cell imaging, cell culture under flow,
--- Diagnostic tech: Diagnostic devices for personalized medicine, novel biosensor research
--- Others: Chemical synthesis and screening, cosmetics, food industry, aerospace and more...


 DATASHEET

 
Specification Info
Number of devices per chip 2 devices
Material PDMS bonded on microscopy glass. Please also check "Our Trip to PDMS Chips" blog post.
Bonding technique Oxygen plasma treatment
XY Size of total chip 25x73 mm2 of chip on 26x76 mm2 of std microscopy glass
Z Height of PDMS chip 3.0 - 4.0 mm
XY Size of micro channels X width is 0.3 mm and Y length is 13.8 mm.
Z Height of micro channels 100 micrometer
Number of solutions 2 solutions for gradient
After mixing reservoir volume 0,75 uL per channel excluding channels
Ports on top/edge on Top
Number of ports 3 ports per device
Pitch btw ports and edge-to-port 3 mm and 3.5 mm
Size / Shape of ports 2 mm / Circle
Suitable connector outer diameter 2.0 - 2.5 mm
microChannel geometry Rectangular
Layout of Active ports small-layout-bw-gr1

 


 REQUIREMENT

Additional Requirements Suggestions
Pumping/Actuation Flow and mixing of the liquids inside microchannels require low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic and piezoelectronic pumps may also be suitable but mind the possible relatively high pulsation.
Fittings All fittings in ufluidic.com are suitable with this chip. Any other connectors compatible with pitch and port size is also applicable. Reactive compability of the fittings is also important to be considered.
Chemicals Depends on the application of researcher.
Visualisation Epi-Fluorescent Microscopy is widely used together with microfluidic chips.
Other analysis techniques should be determined by user researchers for the experiments where optical changes inside chips do not occur.

 


 LITERATURE

Application examples from literature

These are some highly sited articles from literature. The chip designs are not identical but working is same in principle.

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