DG-DropSeq-Z120 Droplet Microfluidics Chip
THIS CHIP IS THE ORIGINAL DESIGN OF THE DROPSEQ TECHNOLOGY, INITIALLY PUBLISHED BY MACOSKO et.al. (2015) IN CELL. DROP-SEQ TECHNOLOGY ENABLES RESEARCHERS TO DO SINGLE CELL LEVEL RNA SEQUENCING AND EXPRESSION LEVEL ANALYSIS.
FOR MORE INFORMATION, PLEASE CHECK THE WEBSITE OF MCCAROLL LAB.
ALL PRICES ARE WITHOUT VAT AND THE SHIPPING COSTS CAN BE SEEN AT THE SHOPPING CART AFTER PROVIDING SHIPPING ADDRESS.
Drop-seq technology enables researchers to do single-cell level RNA sequencing and expression level analysis. For the complete system of single-cell RNA analysis please refer to the authors' website. [[[McCarollLab.]]].
SINCE THIS IS A STATE-OF-ART CONTRACTUAL PRODUCT, THE USERS SHALL GIVE CITATION WHEN THE RESEARCH, IN WHICH THE CHIPS ARE USED, IS PUBLISHED.
|Number of devices per chip||7 devices|
|Droplet size diameter||It is suitable for 120 micrometers (um). Larger droplets are elliptic.|
|Material||PDMS bonded on microscopy glass. Please also check the "Our Trip to PDMS Chips" blog post.|
|Bonding Technique||Oxygen plasma treatment|
|XY Size of total chip||25x73 mm2 of a chip on 26x76 mm2 of std microscopy glass|
|Z Height of microchannels||120 micrometer|
|Z Height of PDMS chip||3.0 - 4.0 mm|
|Ports on top/edge||on Top|
|Number of ports||4 ports per device|
|Pitch btw ports and edge-to-port||NA|
|Size / Shape of ports||1 mm / Circle|
|Suitable Connector Outer Diameter||1.0 - 2.0 mm|
|The layout of active ports||NA; Not applicable|
|Pumping/Actuation||Droplet generation requires low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic, piezo electronic pumps are not suggested due to relatively high pulsation.
|Fittings||All fittings in ufluidic.com are suitable for this chip. Any other connectors compatible with pitch and port size are also applicable.|
|Chemicals||Samples of oil and water phases must be used together in droplet chips.
The surfactant in the oil phase is necessary to obtain stable droplets.
Fluorinated oil and surfactants are mostly accepted for cell culturing studies inside droplets generated.
As the water phase, most cell culture mediums and ionic buffers are acceptable.
|Visualization||Epi-Fluorescent Microscopy is widely used together with droplet generation chips.
Since droplet generation occurs at high speed inside microchannels, a recording may require high-fps cameras or photosensors regarding the application.
These are some highly sited articles from literature. The chip designs are IDENTICAL and working is the same in principle.
|Macosko et.al. 2015|
|Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets|
|Kullanım Kılavuzu||Yakında eklenecektir|
|Nasıl Kullanılır - Multimedya||Yakında eklenecektir|
|Akış Testi - Multiedya||
|Damlacık Oluşum Testi - Multimedya||Yakında elenecektir|