DG-DropSeq-Z120 Droplet Microfluidics Chip

State of the Art

  • €89,00
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THIS CHIP IS THE ORIGINAL DESIGN OF THE DROPSEQ TECHNOLOGY, INITIALLY PUBLISHED BY MACOSKO et.al. (2015) IN CELL. DROP-SEQ TECHNOLOGY ENABLES RESEARCHERS TO DO SINGLE CELL LEVEL RNA SEQUENCING AND EXPRESSION LEVEL ANALYSIS.
FOR MORE INFORMATION, PLEASE CHECK THE WEBSITE OF MCCAROLL LAB.

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Drop-seq technology enables researchers to do single-cell level RNA sequencing and expression level analysis. For the complete system of single-cell RNA analysis please refer to the authors' website. [[[McCarollLab.]]].

SINCE THIS IS A STATE-OF-ART CONTRACTUAL PRODUCT, THE USERS SHALL GIVE CITATION WHEN THE RESEARCH, IN WHICH THE CHIPS ARE USED, IS PUBLISHED.

 


 DATASHEET
 
Specification Info
Number of devices per chip 7 devices
Droplet size diameter It is suitable for 120 micrometers (um). Larger droplets are elliptic.
Material PDMS bonded on microscopy glass. Please also check the "Our Trip to PDMS Chips" blog post.
Bonding Technique Oxygen plasma treatment
XY Size of total chip 25x73 mm2 of a chip on 26x76 mm2 of std microscopy glass
Z Height of microchannels 120 micrometer
Z Height of PDMS chip 3.0 - 4.0 mm
Ports on top/edge on Top
Number of ports 4 ports per device
Pitch btw ports and edge-to-port NA
Size / Shape of ports 1 mm / Circle
Suitable Connector Outer Diameter 1.0 - 2.0 mm
microChannel geometry Rectangular
The layout of active ports NA; Not applicable
... ...

 


 REQUIREMENTS

Additional Requirements Suggestions
Pumping/Actuation Droplet generation requires low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic, piezo electronic pumps are not suggested due to relatively high pulsation.
Fittings All fittings in ufluidic.com are suitable for this chip. Any other connectors compatible with pitch and port size are also applicable.
Chemicals Samples of oil and water phases must be used together in droplet chips.
The surfactant in the oil phase is necessary to obtain stable droplets.
Fluorinated oil and surfactants are mostly accepted for cell culturing studies inside droplets generated.
As the water phase, most cell culture mediums and ionic buffers are acceptable.
Visualization Epi-Fluorescent Microscopy is widely used together with droplet generation chips.
Since droplet generation occurs at high speed inside microchannels, a recording may require high-fps cameras or photosensors regarding the application.

 


 LITERATURE

Application examples from literature

These are some highly sited articles from literature. The chip designs are IDENTICAL and working is the same in principle.

Macosko et.al. 2015
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
DOI: 10.1016/j.cell.2015.05.002

 

 


 
Kullanım Kılavuzu Yakında eklenecektir
Nasıl Kullanılır - Multimedya Yakında eklenecektir
Akış Testi - Multiedya
Damlacık Oluşum Testi - Multimedya Yakında elenecektir

 

 



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