DG4-100um-Z500 Droplet Microfluidic Chips

NBT Original

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Product Type - PDMS on Glass
By NBT Original
Single Cell Analysis
Micro Encapsulation





In microfludics, generally speaking, there are 3 types of flow; laminar, droplet, and digital. Laminar flow, is well-known in micro-sized channels due to Reynold's number calculation. Digital and droplet flow conditions are similar to each other but in digital manipulation of liquid in microfludics, embedded electrodes and hydrophobic surfaces are used to move, mix or seperate droplets of liquid samples individually. Droplet flow, by the way, is a very beneficial finding in microfluidics tech.

Two immiscible liquid flowing opposite in a cross-section of microchannels do not mix with each other, obviously, and one forms micro-sized vesicles inside the other. The droplet formation depends on the cross-sectin geometry, channel sizes, relative flow rates, interfacial tension, and for some time the whole setup of tubings and pumping. The continuous phase in droplet microfluidics, is the liquid in which droplets are formed and the dispersed phase is the liquid of droplets. Using oil-water droplets is very common and mostli w-in-o droplets are used in Life Science applications. w-in-o-in-w double droplet applications or hydrogel-in-oil applications also become popular.

This is a passive droplet generation chip but there are also active (by electrical, magnetic, or centrifugel) droplet formation chip techniques. Although this chip is for passive droplet formation the actuation of liquid flow requires a pumping system.

Droplet microfluidics is accepted by various scientific and industrial applications mainly due to its highly monodisperse, well-controlled production and being high-throughput. These are some application fields:

--- Life Sciences: Single-cell pharmaceutical effect analysis, single cell gene expression level analysis, single cell mRNA/DNA sequencing analysis, digital droplet PCR, protein crystallization and screening,
--- Parmaceutical Sciences: Pharmaceutical synthesis and screening, pharmaceutical encapsulation inside microparticles / micro-bubble / micro-capsule formations, magnetic bead formation
--- Biotechnology: Therapeutical particle synthesis, single cell DNA modification analysis and screening, metagenomic screening, molecular library formation and screening, Bio-printing of cell spheres,
--- Diagnostic tech: Diagnostic devices for personalized medicine, novel biosensor research
--- Others: Chemical synthesis and screening, cosmetics, food industry, aerospace and more...


Specification Info
Number of devices per chip 4 devices
Droplet size diameter It is suitable for 100-500 micrometers (um). Larger droplets are elliptic.
Material PDMS bonded on microscopy glass. Please also check "Our Trip to PDMS Chips" blog post
Bonding Technique Oxygen plasma treatment
XY Size of total chip 25x73 mm2 of chip on 26x76 mm2 of std microscopy glass
Z Height of microchannels 500 micrometer
Z Height of PDMS chip 3.0 - 4.0 mm
Ports on top/edge on Top
Number of ports 5 ports per device
Pitch btw ports and edge-to-port 3 mm and 3.5 mm
Size / Shape of ports 2 mm / Circle
Suitable Connector Outer Diameter 2.0 - 2.5 mm
microChannel geometry Rectangular
Layout of active ports small-layout-bw-dg



Additional Requirements Suggestions
Pumping/Actuation Droplet generation requires low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic, piezoelectronic pumps are not suggested due to relatively high pulsation.
Fittings All fittings in ufluidic.com are suitable with this chip. Any other connectors compatible with pitch and port size is also applicable.
Chemicals Samples of oil and water phases must be used together in droplet chips.
Surfactant in oil phase is necessary to obtain stable droplets.
Flourinated oil and surfactants are mostly accepted for cell culturing studies inside droplets generated.
As water phase, most cell culture mediums and ionic buffers are acceptable.
Visualisation Epi-Fluorescent Microscopy is widely used together with droplet generation chips.
Since, droplet generation occurs at high speed inside microchannels, recording may require high-fps cameras or photosensors regarding the application.



Application examples from literature

These are some highly sited articles from literature. The chip designs are not identical but working is same in principle.

Adam et.al. 2019
Droplet microfluidics for generation of granular matters and functional elements
doi: 10.14356/kona.2019004
Rakszewska et.al. 2014
One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis
doi: 10.1038/am.2014.86
Ostafe et.al. 2014
A high-throughput cellulase screening system based on droplet microfluidics
doi: 10.1063/1.4886771
 Li et.al. 2018
Microfluidic fabrication of microparticles for biomedical applications
doi: 10.1039/C7CS00263G
MacConnell et.al. 2017
An Integrated Microfluidic Processor for DNA-Encoded Combinatorial Library Functional Screening
doi: 10.1021/acscombsci.6b00192
Shembekar et.al. 2018
Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells
doi: 10.1016/j.celrep.2018.01.071
Kwak et.al. 2018
Mass fabrication of uniform sized 3D tumor spheroid using high-throughput microfluidic system
doi: 10.1016/j.jconrel.2018.02.029
Hou et.al. 2018
Injectable degradable PVA microgels prepared by microfluidic technology for controlled osteogenic differentiation of mesenchymal stem cells
doi: 10.1016/j.actbio.2018.07.003
Abalde-Cela et.al. 2018
Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles
doi: 10.1038/s41598-018-20754-x

 Kullanım Kılavuzu  Yakında eklenecektir 
 Nasıl kullanılır - Multimedya   Yakında eklenecektir 
 Akış Testleri - Multimedya   Yakında eklenecektir 
 Droplet Oluşumu - Multimedya   Yakında eklenecektir 

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