HDF2-Z100 HydroDynamic Focusing Chip
THIS IS A Y-TYPE HYDRODYNAMIC FOCUSING CHIP MICROFLUIDIC CHIP. THE 3 TYPES OF LIQUIDS, MOSTLY SAMPLES ON THE CENTER AND CLEAN BUFFERS ON SIDES ARE PUMPED SO AT THE CROSSING POINT THEY DO NOT MIX BUT REGARDING RELATIVE VELOCITIES OR PRESSURES THE MIDDLE SAMPLE LIQUID IS SQUEEZED IN BETWEEN.
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Microfluidics is beneficial for saving reagent consumption. Hydro Dynamic Focusing devices are suitable for the applications below;
--- Life Sciences: ...
--- Pharmaceutical Sciences: ...
--- Biotechnology: ...
--- Diagnostic tech: ...
--- Others: ...
|Number of devices per chip||4 devices|
|Material||PDMS bonded on microscopy glass. Please also check the "Our Trip to PDMS Chips" blog post.|
|Bonding technique||Oxygen plasma treatment|
|XY Size of total chip||25x73 mm2 of the chip on 26x76 mm2 of std microscopy glass|
|Z Height of PDMS chip||3.0 - 4.0 mm|
|XY Size of microchannels||X width is 0.3 mm and Y length is 18.0 mm.|
|Z Height of microchannels||100 micrometer|
|Number of solutions||3 solutions of which at the center is squeezed in between|
|After crossing channel XY size||X width is 0.3 mm and Y length is 6.5 mm.|
|Ports on top/edge||on Top|
|Number of ports||4 ports per device|
|Pitch btw ports and edge-to-port||3 mm and 3.5 mm|
|Size / Shape of ports||2 mm / Circle|
|Suitable connector outer diameter||2.0 - 2.5 mm|
|The layout of Active ports||.|
|Pumping/Actuation||Flow and mixing of the liquids inside microchannels require low-pulsation flow.
Some high-quality syringe pumps and pressure pumps work well.
Peristaltic and piezoelectric pumps may also be suitable but mind the possible relatively high pulsation.
|Fittings||All fittings in ufluidic.com are suitable with this chip. Any other connectors compatible with pitch and port size is also applicable. Reactive compatibility of the fittings is also important to be considered.|
|Chemicals||Depends on the application of the researcher.|
|Visualization||Epi-Fluorescent Microscopy is widely used together with microfluidic chips.
Other analysis techniques should be determined by user researchers for the experiments where optical changes inside chips do not occur.
These are some highly sited articles from literature. The chip designs are not identical but working is the same in principle.
|Hydrodynamic Focusing in Microfluidic Devices|
|Mengqian Lu et.al., 2016|
|Microfluidic hydrodynamic focusing for synthesis of nanomaterials|
|Sima Rezvantalab and Mostafa Moraveji, 2019|
|Microfluidic assisted synthesis of PLGA drug delivery systems|
|Jin Akagi et.al., 2013|
|Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry|
|Kullanım Kılavuzu||Yakında eklenecektir|
|Nasıl kullanılır - Multimedya||Yakında eklenecektir|
|Akış Testi - Multimedya||Yakında eklenecektir|