HDF3-Z100 HydroDynamic Focusing Chip

THIS IS A FLOW FOCUSING-TYPE HYDRODYNAMIC FOCUSING CHIP. THE 2 TYPES OF LIQUIDS, MOSTLY SAMPLES ON THE CENTER AND CLEAN BUFFERS ON SIDES ARE PUMPED SO AT THE CROSSING POINT THEY DO NOT MIX BUT REGARDING RELATIVE VELOCITIES OR PRESSURES THE MIDDLE SAMPLE LIQUID IS SQUEEZED IN BETWEEN.

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• DESCRIPTION
• DATASHEET
• REQUIREMENTS
• LITERATURE

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Microfluidics is beneficial for saving reagent consumption. Hydro Dynamic Focusing devices are suitable for the applications below;

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 DATASHEET

Specification	Info
Number of devices per chip	5 devices
Material	PDMS bonded on microscopy glass. Please also check the "Our Trip to PDMS Chips" blog post.
Bonding technique	Oxygen plasma treatment
XY Size of total chip	25x73 mm2 of the chip on 26x76 mm2 of std microscopy glass
Z Height of PDMS chip	3.0 - 4.0 mm
XY Size of microchannels	X width is 0.3 mm and Y length is 18.0 mm.
Z Height of microchannels	100 micrometer
Number of solutions	3 solutions of which at the center is squeezed in between
After crossing channel XY size	X width is 0.3 mm and Y length is 6.5 mm.
Ports on top/edge	on Top
Number of ports	4 ports per device
Pitch btw ports and edge-to-port	3 mm and 3.5 mm
Size / Shape of ports	2 mm / Circle
Suitable connector outer diameter	2.0 - 2.5 mm
microChannel geometry	Rectangular
The layout of Active ports

 

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 REQUIREMENTS

Additional Requirements	Suggestions
Pumping/Actuation	Flow and mixing of the liquids inside microchannels require low-pulsation flow. Some high-quality syringe pumps and pressure pumps work well. Peristaltic and piezoelectric pumps may also be suitable but mind the possible relatively high pulsation.
Fittings	All fittings in ufluidic.com are suitable with this chip. Any other connectors compatible with pitch and port size is also applicable. Reactive compatibility of the fittings is also important to be considered.
Chemicals	Depends on the application of the researcher.
Visualization	Epi-Fluorescent Microscopy is widely used together with microfluidic chips. Other analysis techniques should be determined by user researchers for the experiments where optical changes inside chips do not occur.

 

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 LITERATURE

Application examples from literature

These are some highly sited articles from literature. The chip designs are not identical but working is the same in principle.

Dziubinski, 2011

Hydrodynamic Focusing in Microfluidic Devices

DOI: 10.5772/34690

Mengqian Lu et.al., 2016

Microfluidic hydrodynamic focusing for synthesis of nanomaterials

DOI: 10.1016/j.nantod.2016.10.006

Sima Rezvantalab and Mostafa Moraveji, 2019

Microfluidic assisted synthesis of PLGA drug delivery systems

DOI: 10.1039/C8RA08972H

Jin Akagi et.al., 2013

Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry

DOI: 10.1117/12.2001474